Cilia are highly conserved organelles that play various motile and sensory functions in many eukaryotes. For example, cilia are involved in cell motility, sensory perception, movement of extra-cellular fluids and determination of left-right asymmetry. In humans, defects in cilia assembly or function are responsible for a wide range of diseases called ciliopathies. Our team seeks to understand how cilia are assembled during animal development and what governs ciliary diversity within an organism, using Drosophila as a model system. Our team also investigates how, in the absence of cilia or centrioles, the muscle cell organizes its cytoskeleton network from nuclei and what is the role of the proteins associated with cilia and centrioles in muscle differentiation and function.
For this we combine advanced imaging approaches (high resolution, electronic, real-time confocal) and functional genetics using the extraordinary panoply of tools for genome manipulation or gene expression available in Drosophila (RNAi, site specific recombination, CrispR /Cas9 induced genome manipulation).We develop :
(1) Genetic screens by RNAi in Drosophila for genes required for the conversion of centrioles into basal bodies.
(2) Biochemical screens in Drosophila and mammalian cells for proteins interacting with specific components of the ciliary base or of the centrioles, using the Biotinylation Proximity Labeling System (BioID-Apex Methodology).
(3) An RNAi screen for interactors of the nesprin1 gene, encoding a protein of the nuclear membrane that is involved in cytoskeleton organization in muscle cells.
These different axes, will allow, on the one hand, to better understand the mechanisms that allow the conversion of centrioles into basal bodies and which govern the formation of different ciliary types and, on the other hand, to understand how the muscle cell is freed from the loss of centrioles in the organization of its cytoskeletal network.
- Transition zone assembly and its contribution to axoneme formation in Drosophila male germ cells
Vieillard J., Paschaki M., Duteyrat JL, Augière C., Cortier E., Lapart J.A., Thomas J. and Durand B. JCB 2016 (214: 875-889) .
- Drosophila melanogaster as a model for basal body research
Jana S.C., Bettencourt-Dias M., Durand B., and Megraw T.L. Cilia (2016) 5:22.
- RFX2 Is a Major Transcriptional Regulator of Spermiogenesis
Kistler W.S., Baas D., Lemeille S., Paschaki M., Seguin-Estevez Q., Barras E., Ma W., Duteyrat J.L., Morlé L., Durand B. and Reith W. Plos Genetics (2015) e1005368.
- Imaging cilia in Drosophila melanogaster
Vieillard J., Duteyrat J.L., Cortier E. and Durand B. Methods Cell Biol. (2015) 127:279-302.
- Drosophila Nesprin-1 controls glutamate receptor density at neuromuscular junctions
Morel V., Lepicard S., Rey A.N., Parmentier M.-L., and Schaeffer L. Cell. Mol. Life Sci. (2014) 71:3363–3379.
- The coiled-coiled domain containing protein CCDC151 is required for efficient motility of IFT dependant cilia in animals
Jerber J., Baas D., Soulavie F., Chhin B., Cortier E., Vesque C., Thomas J. and Durand B. Human Molecular Genetics (2014) 23 :563-77.
- hemingway is required for axonemal stability and ciliary motility in Drosophila
Soulavie F., Piepenbrock D., Thomas J., Vieillard J., Duteyrat J.L., Cortier E., Laurençon A., Göpfert M.C. and Durand B. MBoC. (2014) 25 :1276-86.
- Drosophila Chibby is required for basal body formation and ciliogenesis but not for wingless signaling
Enjolras C., Thomas J., Chhin B., Cortier E., Duteyrat JL, Soulavie F., Kernan M.J., Laurençon A. and Durand B. J. Cell Biol. (2012) 197(2):313-25.
- ANR DIVERCIL 2018-2022
- AFM MyoNeurALP