INMG

Institut NeuroMyoGène


CNRS UMR 5310 - INSERM U1217 - Université de Lyon - Université Claude Bernard Lyon 1


Soutenance Viviane Lainé
PhD Defense – Wednesday July 13th 14:00 - Amphithéâtre CNRS

Study of sub-cellular modifications associated to muscle aging in Caenorhabditis elegans - role of the transcription factor UNC-120/SRF

Adeline​ Mergoud dit Lamarche - Directrice de thèse Florence Solari - Équipe Bessereau

Aging is accompanied by a progressive loss of muscle mass and function, named sarcopenia. Different mechanisms have been proposed to explain it.
​However most of them have been identified in the context of an experimentally induced atrophy (by denervation, immobilization, fasting, etc.) or via correlative studies in humans. Thus today we do not know the importance and chronology of these factors in the context of physiological aging.
Caenorhabditis elegans is a reference model organism for longevity studies. Thanks to its short lifespan and to available powerful genetics tools, C. elegansi allowed the identification of conserved molecular pathways , such as the Insulin/IGF-1 receptor pathway, which have been later shown to control longevity also in mammals. However muscle aging has been very poorly studied in this organism.
By taking advantage of this model, the first aim of my (thesis) project was to describe subcellular changes that are associated with the loss of mobility with age, in order to identify potential biomarkers of muscle aging. The second aim was to use these biomarkers as tools to seek for genes that modify sarcopenia.
Specifically, we observed a sharp decrease in the level of mRNA encoding proteins involved in muscle structure and function very early during adulthood. This phenotype is followed by the gradual fragmentation of mitochondria, an later on a increase in autophagic vesicles number.
These biomarkers have been used to test transcription factors known to be involved in muscle differentiation during embryogenesis, for their potential role in sarcopenia. Altogether our results suggest a model in which the transcription factor unc-120, the ortholog of Serum Response Factor/SRF, would act downstream the insulin/IGF-1 receptor DAF-2 to allow muscle maintenance with age.​


More information about the science of team Bessereau

 
 
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Publication

Cytoplasmic NOTCH and membrane-derived β-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis

Daniel Sieiro, Anne C Rios, Claire E Hirst, Christophe Marcelle

How cells in the embryo coordinate epithelial plasticity with cell fate decision in a fast changing cellular environment is largely unknown. In chick embryos, skeletal muscle formation is initiated by migrating Delta1-expressing neural crest cells that trigger NOTCH signaling and myogenesis in selected epithelial somite progenitor cells, which rapidly translocate into the nascent muscle to differentiate. Here, we uncovered at the heart of this response a signaling module encompassing NOTCH, GSK-3β, SNAI1 and β-catenin.


More information about the science of team Marcelle

 
 

Research teams

INMG houses 14 research teams focusing on the physiology of the neuromuscular system, cellular and molecular neurobiology and nuclear dynamics.

News & Highlights

Julien Courchet receives ERC Starting Grant (ERC StG 2015).

Julien Courchet’s group will investigate how the regulation of glucose metabolism and mitochondria function by two kinases termed LKB1 and NUAK1 underlies neuron development and circuit formation in the cortex of the mouse.

Seminars


Tuesday July 12th at 11:00 - Amphithéâtre CNRS
Jan Tuckermann – Institute of Comparative Molecular Endocrinology Ulm University, Ulm, Germany

Job Offers

  • Team Boulin 2 year Post-doctoral position. (C. elegans, cellular excitability, potassium channels)
  • Team Bessereau One Post-doctoral position. (Mechanisms involved in GABAA receptor clustering at C. elegans synapses)
    more info here

Students & Training

L'INMG accueille et forme chaque année de nombreux étudiants à tous les niveaux de formation.

Facilities & Resources

Administration

Director
Pr. Laurent SCHAEFFER

Co-Director - Science policy
Pr. Jean-Louis BESSEREAU

Co-Directeur - Human resources
Dr. Guy MOUCHIROUD

Administrative hub et secretariat
Juliette CAMPANA
Laurence FOURNIER
Isabelle MEZZANO
Isabelle ROMERA
Jean-Sébastien GAILLARD

Research Teams

Students

L'INMG accueille et forme chaque année de nombreux étudiants à tous les niveaux de formation.
 
  • Internships
  • Graduate schools
    • BMIC - École Doctorale de Biologie Moléculaire, Intégrative et Cellulaire de Lyon
    • EDISS - École Doctorale Interdisciplinaire Sciences Santé
    • NSCo - Neurosciences et Cognition

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  • Secrétariat La Doua

    Isabelle MEZZANO
    Tel.: +33 4 72 43 13 93
  • Adresse Campus La Doua

    Institut NeuroMyoGène
    CNRS UMR 5310 - INSERM U1217 - Université Claude Bernard Lyon 1
    16 rue Raphaël Dubois
    69100 Villeurbanne
    France

  • Secrétariat ENS Lyon


    Tel.: +33 4
  • Adresse Campus ENS LYON

    LBMC, UMR5239 ENS Lyon
    46 Allée d'Italie
    69007 LYON
    FRANCE

  • Secrétariat Laennec

    Juliette CAMPANA
    Tel.: +33 4 78 77 87 59
  • Adresse Campus Laënnec

    Faculté de Médecine Lyon Est – Laënnec
    Rez de chaussée Bâtiment B
    7, rue G. Paradin
    69372 LYON Cedex 08
    France