Centrioles and cilia play fundamental roles in cell and tissue homeostasis in animals. Many human pathologies result from defects in cilia assembly or function. While almost all cells harbor cilia during mammalian development, some tissues like skeletal muscles lose their centrioles and organize their microtubules around nuclei. We seek to understand: 1) the mechanisms that govern cilia diversity and their growth from centrioles; 2) how muscle cells organize their microtubule network around the nucleus.
Cilia are highly conserved organelles that play various motile and sensory functions in many eukaryotes. For example, cilia are involved in cell motility, sensory perception, movement of extra-cellular fluids and determination of left-right asymmetry. In humans, defects in cilia assembly or function are responsible for a wide range of diseases called ciliopathies.
Our team seeks to understand how cilia are assembled during animal development and what governs ciliary diversity within an organism, using Drosophila as a model system. Our team also investigates how, in the absence of cilia or centrioles, the muscle cell organizes its cytoskeleton network from nuclei and what is the role of the proteins associated with cilia and centrioles in muscle differentiation and function. For this, we combine advanced imaging approaches (high resolution, electronic, real-time confocal), biochemical screens and functional genetics using the extraordinary panoply of tools for genome manipulation or gene expression available in Drosophila (RNAi, site specific recombination, CrispR /Cas9 induced genome manipulation).
UCBL – CNRS UMR 5310 – INSERM U1217
Faculté de Médecine et de Pharmacie – 3ème étage – Couloir CD
8 avenue Rockefeller