PROJETS

Au delà de la compréhension de la biologie du virus HSV-1, l’équipe développe des recherches qui ont pour but de comprendre au niveau moléculaire et en utilisant le génome du virus HSV-1 comme modèle, certains aspects fondamentaux de la dynamique nucléaire et chromatinienne en relation avec l’intégrité du génome et la régulation de l’expression génique.

SÉLECTION DE PUBLICATIONS

• Corpet, A., Kleijwegt, C., Roubille, S., Juillard, F., Jacquet, K., Texier, P., and Lomonte, P. (2020). PML nuclear bodies and chromatin dynamics: catch me if you can! Nucleic Acids Research 389, 251–23
• Lomonte, P., Baklouti, F., & Binda, O. (2020). The Biochemistry of Survival Motor Neuron Protein Is Paving the Way to Novel Therapies for Spinal Muscle Atrophy. Biochemistry. Apr14;59(14):1391-1397.
• Marcocci ME, Napoletani G, Protto V, Kolesova O, Piacentini R, Li Puma DD, Lomonte P, Grassi C, Palamara AT, De Chiara G. (2020). Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection. Trends in Microbiology. 1-13.
• Cohen, C., Corpet, A., Maroui, M.A., Juillard, F., and Lomonte, P. (2020). Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH). Methods Mol. Biol. 2060, 185–197.
• Provost A., Rousset C.,, Bourdon L., Mezhoud S., Reungoat E., Fourneaux C., Bresson T., Pauly M., Béard N., Possi-Tchouanlong L., Grigorov B., Bouvet P., Diaz J.J., Chamot C., Pécheur E.-I., Ladavière C. *, Charreyre M.-T. *, Favier A., Place C.*, Monier K. *. (2019). Innovative particle standards and long-lived imaging for 2D and 3D dSTORM. Sci. Rep. 9(1):17967
• Grandin N, Pereira B, Cohen C, Billard P, Dehais C, Carpentier C, Idbaih A, Bielle F, Ducray F, Figarella-Branger D, Delattre JY, Sanson M, Lomonte P, Poncet D, Verrelle P, Charbonneau M; POLA network. (2019). The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas.Acta Neuropathol Commun. 7, 175.
• Cohen, C., Corpet, A., Roubille, S., Ali Maroui, M.A., Poccardi, N., Rousseau, A., Kleijwegt, C., Binda, O., Texier, P., Sawtell, N., Labetoulle, M., and Lomonte, P. (2018). Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone axis. Plos Pathogens 14(9): e1007313.
• Herpesvirus latency: On the importance of positioning oneself
  Lomonte P. Advances in Anatomy, Embryology and Cell Biology. Cell Biology of Herpes Viruses. Ed. Springer Nature (2017) vol. 223, pp95–117.
• The interaction between herpes simplex virus 1 genome and promyelocytic leukemia nuclear bodies (PML-NBs) as a hallmark of the entry in latency
  Lomonte P. Microbial Cell (2016) vol. 3 (11), pp. 438-441.
• Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment
  Maroui M-A*, Callé A*, Cohen C, Streichenberger N, Texier P, Takissian J, Rousseau A, Poccardi N, Welsch J, Corpet A, Schaeffer L, Labetoulle M, Lomonte P. Plos Pathogens (2016) 12: e1005834.
• Real-time tracking of parental histones reveals their contribution to chromatin integrity followingDNA damage
  Adam S, Dabin J, Chevallier O, Leroy O, Baldeyron C, Corpet A, Lomonte P, Renaud O, Almouzni G, Polo SE. Molecular Cell (2016) vol. 64 (1) pp. 65-78.
• Establishment of HSV1 latency in immunodeficient mice facilitates efficient in vivo reactivation
  Ramakrishna C, Ferraioli A, Calle A, Nguyen TK, Openshaw H, Lundberg PS, Lomonte P, Cantin EM. Plos pathogens (2015) Mar 11;11(3):e1004730.
• Expression of the epidermodysplasia verruciformis-associated genes EVER1 andEVER2 is activated by exogenous DNA and inhibited by LMP1 oncoprotein fromEpstein-Barr virus
  Frecha C, Chevalier S, van Uden P, Rubio I, Siouda M, Saidj D, Cohen C, Lomonte P, Accardi R, and Tommasino M. J virol (2015) Jan 15;89(2):1461-7.
• Biological features of herpes simplex virus type 1 latency in mice according to experimental conditions and type of neurones
  Cavallero S, Huot N, Francelle L, Lomonte P, Naas T, Labetoulle M. Invest Ophthalmol Vis Sci. (2014) Oct 16;55(12):7761-74. .
• Corps nucléaires PML, centromères et contrôle de la latence du virus herpès simplex 1
  Lomonte P. Virologie (2014) 18 (3), 170-179.
• Detection of the genome and transcripts of a persistent DNA virus in neuronal tissues by fluorescent in situ hybridization combined to immuno-staining
  Catez F, Rousseau A, Labetoulle M, and Lomonte P. Journal of Visualized Experiment, JoVE (2014) JoVE, (83), e51091.
• The Tudor protein survival motor neuron (SMN) is a chromatin-binding protein that interacts with methylated lysine 79 of histone H3
  Sabra M, Texier P, El Maalouf J, and Lomonte P. Journal of Cell Science (2013) 126 (Pt 16) pp. 3664-3677.

FINANCEMENTS

• Programme prématuration du CNRS : imagerie dSTORM dans le canal vert pour reconstruire 3 couleurs et 3D en super-résolution dSTORM (18 mois)
• ANR CHROMACoV (ANR-20-COV9-0004)
• ANR EPIPRO (ANR-18-CE15-0014-01)
• LabEX DEV2CAN (ANR-10-LABX-61)
• LabEX DEVweCAN (ANR-10-LABX-61)
• AFM-Téléthon / MyoNeurALP Alliance (2016-2021)
• France Alzheimer
• Fondation pour la Recherche Médicale (FRM)
• Ligue Contre le Cancer (Comité du Rhône)
• UCBL: CNMD (Ottawa, Canada)-INMG Joint collaborative Research Program