- The major research interests of the team LOMONTE are:
- The study of the pathological consequences of the infection of neurons from the peripheral and central nervous systems by the neurotropic virus herpes simplex virus 1 (HSV-1). We essentially focus our research on the establishment of latency and reactivation processes.
- The study of the implication of nuclear architecture, especially promyelocytic leukemia nuclear bodies (PML NBs), in the regulation of latent HSV-1 chromatin features and in chromatin dynamics during the process of senescence.
- The study of the chromatin alterations associated to damage or to genetic pathologies, and potentially linked to genetic instabilities, or motoneurons decay.
TEAM
- Patrick LOMONTE
SENIOR RESEARCHER CNRS - Faouzi BAKLOUTI
SENIOR RESEARCHER INSERM - Olivier BINDA
POST-DOC - Armelle CORPET
ASSISTANT PROFESSOR UCBL - Karine JACQUET
POST-DOC - Franceline JUILLARD
POST-DOC - Constance KLEIJWEGT
PHD STUDENT - Simon ROUBILLE
PHD STUDENT - Pascale TEXIER
RESEARCH ASSISTANT CNRS
PROJECTS
Beside the study of the biology of HSV-1, one of the main focus of the team is to understand, at the molecular level and using HSV-1 as a model, some basic aspects of nuclear and chromatin dynamics related to the maintenance of genome integrity and to the regulation of gene expression. (See web site for more details)
SELECTED PUBLICATIONS
- Lomonte, P., Baklouti, F., & Binda, O. (2020). The Biochemistry of Survival Motor Neuron Protein Is Paving the Way to Novel Therapies for Spinal Muscle Atrophy. Biochemistry. Apr14;59(14):1391-1397.
- Marcocci ME, Napoletani G, Protto V, Kolesova O, Piacentini R, Li Puma DD, Lomonte P, Grassi C, Palamara AT, De Chiara G. (2020). Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection. Trends in Microbiology. 1-13.
- Cohen, C., Corpet, A., Maroui, M.A., Juillard, F., and Lomonte, P. (2020). Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH). Methods Mol. Biol. 2060, 185–197.
- Grandin N, Pereira B, Cohen C, Billard P, Dehais C, Carpentier C, Idbaih A, Bielle F, Ducray F, Figarella-Branger D, Delattre JY, Sanson M, Lomonte P, Poncet D, Verrelle P, Charbonneau M; POLA network. (2019). Acta Neuropathol Commun. 7, 175.
- Cohen, C., Corpet, A., Roubille, S., Ali Maroui, M.A., Poccardi, N., Rousseau, A., Kleijwegt, C., Binda, O., Texier, P., Sawtell, N., Labetoulle, M., and Lomonte, P. (2018). Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone axis. Plos Pathogens 14(9): e1007313.
- Herpesvirus latency: On the importance of positioning oneself
Lomonte P. Advances in Anatomy, Embryology and Cell Biology. Cell Biology of Herpes Viruses. Ed. Springer Nature (2017) vol. 223, pp95–117. - The interaction between herpes simplex virus 1 genome and promyelocytic leukemia nuclear bodies (PML-NBs) as a hallmark of the entry in latency
Lomonte P. Microbial Cell (2016) vol. 3 (11), pp. 438-441. - Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment
Maroui M-A*, Callé A*, Cohen C, Streichenberger N, Texier P, Takissian J, Rousseau A, Poccardi N, Welsch J, Corpet A, Schaeffer L, Labetoulle M, Lomonte P. Plos Pathogens (2016) 12: e1005834. - Real-time tracking ofparental histones reveals their contribution to chromatin integrity followingDNA damage
Adam S, Dabin J, Chevallier O, Leroy O, Baldeyron C, Corpet A, Lomonte P, Renaud O, Almouzni G, Polo SE. Molecular Cell (2016) vol. 64 (1) pp. 65-78. - Establishment of HSV1 latency in immunodeficient mice facilitates efficient in vivo reactivation
Ramakrishna C, Ferraioli A, Calle A, Nguyen TK, Openshaw H, Lundberg PS, Lomonte P, Cantin EM. Plos pathogens (2015) Mar 11;11(3):e1004730. - Expression of the epidermodysplasia verruciformis-associated genes EVER1 andEVER2 is activated by exogenous DNA and inhibited by LMP1 oncoprotein fromEpstein-Barr virus
Frecha C, Chevalier S, van Uden P, Rubio I, Siouda M, Saidj D, Cohen C, Lomonte P, Accardi R, and Tommasino M. J virol (2015) Jan 15;89(2):1461-7. - Biological features of herpes simplex virus type 1 latency in mice according to experimental conditions and type of neurones
Cavallero S, Huot N, Francelle L, Lomonte P, Naas T, Labetoulle M. Invest Ophthalmol Vis Sci. (2014) Oct 16;55(12):7761-74. . - Corps nucléaires PML, centromères et contrôle de la latence du virus herpès simplex 1
Lomonte P. Virologie (2014) 18 (3), 170-179. - Detection of the genome and transcripts of a persistent DNA virus in neuronal tissues by fluorescent in situ hybridization combined to immuno-staining
Catez F, Rousseau A, Labetoulle M, and Lomonte P. Journal of Visualized Experiment, JoVE (2014) JoVE, (83), e51091. - The Tudor protein survival motor neuron (SMN) is a chromatin-binding protein that interacts with methylated lysine 79 of histone H3
Sabra M, Texier P, El Maalouf J, and Lomonte P. Journal of Cell Science (2013) 126 (Pt 16) pp. 3664-3677.
FUNDING
- ANR EPIPRO (ANR-18-CE15-0014-01)
- ANR : LabEX DEVweCAN (ANR-10-LABX-61)
- Ligue Contre le Cancer (Comité du Rhône)
- AFM-Téléthon / MyoNeurALP Alliance (2016-2021)
- UCBL: CNMD (Ottawa, Canada)-INMG Joint collaborative Research Program
