Institut NeuroMyoGène
    CNRS UMR 5310 - INSERM U1217
    Université de Lyon
    Université Claude Bernard Lyon 1
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CASTELLANICASTELLANICASTELLANICASTELLANI
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NEURO-DEVELOPMENT, PEDIATRIC CANCER, AND SIGNALING

We investigate the development of the nervous system in the embryo and its deregulation in the context of pediatric cancers.

neuronal development topographic signaling axon guidance cell division cell migration tumorigenesis metastasis

© Frédérique PLAS / INMG / CNRS Photothèque

Team
  • Valérie CASTELLANI
    DR CNRS, HDR
  • Maëlys ANDRE
    DOCTORANT
  • Dounia BEN AMAR
    DOCTORANT
  • Muriel BOZON
    AI CNRS
  • Sophie CALVET
    POST-DOCTORANT
  • Céline DELLOYE-BOURGEOIS
    CR CNRS
  • Hugo DUCUING
    POST-DOCTORANT
  • Olivier IMBAUD
    IE BIOINFO
  • Julien FALK
    CR CNRS, HDR
  • Karine KINBEITER
    IE
  • Frédéric MORET
    MCU UCBL, HDR
  • Florie REYNAUD
    IR
  • Servane TAUSZIG-DELAMASURE
    DR CNRS, HDR
  • Karine THOINET
    AI CNRS
Alumni
  • Leila BOUBAKAR
    DOCTORANT
  • Edmund DERRINGTON
    PU UCBL, HDR
  • Sarah DINVAUT
    DOCTORANT
  • Katrin GERTSMANN
    POST-DOCTORANT
  • Aurora PIGNATA
    DOCTORANT
  • Thibault GARDETTE
    DOCTORANT

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PROJECTS

Polarization, which confers asymmetry at molecular, cellular and tissue scales, is a fundamental process establishing key features of biological systems. In multicellular organisms, symmetry breaking triggers the specification of embryonic body axes, providing general landmarks for subsequent morphogenetic programs. Cells and tissues acquire complex morphological and functional polarity features, which, remarkably, are highly precisely positioned within the general body landmarks. The lab is interested in understanding the mechanisms setting the spatial orientation of some selected polarity processes, taking place during the formation of the nervous system. We investigate the topography of progenitor cell divisions, and neuronal differentiation, using the developing spinal cord and sensorimotor circuits as a model. We also investigate how topographic cues impact on  closely related pathological contexts such as the neuroblastoma, a neuro-developmental cancer arising from trunk neural cell crest, and the medulloblastoma arising from cerebellar progenitors . We exploit chick and mouse embryo animal models, combined with live imaging, cell and tissue cultures and molecular approaches.

 


START UP

Oncofactory is a start-up founded in 2016 as a spin-off of the Castellani lab, based on the work of Dr. Céline Delloye Bourgeois and Dr. Valérie Castellani at the NeuroMyoGene Institute in Lyon. Oncofactory offers an innovative in vivo platform suited for all cancers consisting in the creation of miniaturized replicas of patient tumors in an embryonic organism. Tumors are analyzed in the whole organism using 3D microscopy. Replicas can be collected back for a range of large scale molecular analysis. Using its unique technological platform Oncofactory evaluates the efficacy,  studies the mechanisms of action of therapeutic candidates, helps the design of combi-therapies through a dedicated program combining transcriptomic analysis and its model of patient tumors  replicas.

https://www.oncofactory.com/


SELECTED PUBLICATIONS
  • Spatial and temporal profiling of receptor membrane insertion controls commissural axon responses to midline repellents.
    Pignata A, Ducuing H, Boubakar L, Gardette T, Kindbeiter K, Bozon M, Tauszig-Delamasure S, Falk J, Thoumine O, Castellani V. Cell Rep. (2019) 29, 347–362, October 8.
  • Hijacking of Embryonic Programs by Neural Crest-Derived Neuroblastoma: From Physiological Migration to Metastatic Dissemination.
    Delloye-Bourgeois C, Castellani V. Front Mol Neurosci. (2019) Mar 1;12:52.
  • Septin functions during neuro-development, a yeast perspective.
    Falk J, Boubakar L, Castellani V. Curr Opin Neurobiol. (2019) Feb 18;57:102-109
  • Commissural axon navigation in the spinal cord: A repertoire of repulsive forces is in command
    Ducuing H, Gardette T, Pignata A, Tauszig-Delamasure S, Castellani V. Semin Cell Dev Biol. (2019) S1084-9521:30286-0.
  • Microenvironment-Driven Shift of Cohesion/Detachment Balance within Tumors Induces a Switch toward Metastasis in Neuroblastoma
    Delloye-Bourgeois C, Bertin L, Thoinet K, Jarrosson L, Kindbeiter K, Buffet T, Tauszig-Delamasure S, Bozon M, Marabelle A, Combaret V, Bergeron C, Derrington E, Castellani V. Cancer Cell (2017) 32:427-443.
  • Molecular Memory of Morphologies by Septins during Neuron Generation Allows Early Polarity Inheritance
    Boubakar L, Falk J, Ducuing H, Thoinet K, Reynaud F, Derrington E, Castellani V. Neuron (2017) 95:834-851.
  • Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation
    Charoy C, Dinvaut S, Chaix Y, Morlé L, Sanyas I, Bozon M, Kindbeiter K, Durand B, Skidmore JM, De Groef L, Seki M, Moons L, Ruhrberg C, Martin JF, Martin DM, Falk J, Castellani V. Elife (2017) e18481.
  • Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis
    Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, Pierani A, Falk J, Moret F, Castellani V. Nat Comm (2015) 27;6:6366.
  • PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments
    Delloye-Bourgeois C, Jacquier A, Charoy C, Reynaud F, Nawabi H, Thoinet K, Kindbeiter K, Yoshida Y, Zagar Y, Kong Y, Jones YE, Falk J, Chédotal A, Castellani V. Nat Neurosci. (2014) 18:36-45.
  • Use of pHluorin to assess the dynamics of axon guidance receptors in cell culture and in the chick embryo
    Delloye-Bourgeois C, Jacquier A, Falk J, Castellani V. JOVE (2014) 12:e50883.
  • gdnf activates midline repulsion by Semaphorin3B via NCAM during commissural axon guidance
    Charoy C, Nawabi H, Reynaud F, Derrington E, Bozon M, Wright K, Falk J, Helmbacher F, Kindbeiter K, Castellani V.Neuron (2012) 75:1051-66.

FUNDING

 


PAST FUNDING

Email

valerie.castellani@univ-lyon1.fr

Téléphone

+33 4 26 68 82 50

Adresse

Institut NeuroMyoGène
UCBL – CNRS UMR 5310 – INSERM U1217
Faculté de Médecine et de Pharmacie – 3ème étage – Couloir A
8 Avenue Rockefeller
F-69008 Lyon


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